Wednesday, January 25, 2012

FACT CHECK: Obama pushes plans that flopped before

President Barack Obama delivers his State of the Union address on Capitol Hill in Washington, Tuesday, Jan. 24, 2012. Listen in back are Vice President Joe Biden and House Speaker John Boehner, right. (AP Photo/Saul Loeb, Pool)

President Barack Obama delivers his State of the Union address on Capitol Hill in Washington, Tuesday, Jan. 24, 2012. Listen in back are Vice President Joe Biden and House Speaker John Boehner, right. (AP Photo/Saul Loeb, Pool)

(AP) ? It was a wish list, not a to-do list.

President Barack Obama laid out an array of plans in his State of the Union speech as if his hands weren't so tied by political realities. There can be little more than wishful thinking behind his call to end oil industry subsidies ? something he could not get through a Democratic Congress, much less today's divided Congress, much less in this election year.

And there was more recycling, in an even more forbidding climate than when the ideas were new: He pushed for an immigration overhaul that he couldn't get past Democrats, permanent college tuition tax credits that he asked for a year ago, and familiar discouragements for companies that move overseas.

A look at Obama's rhetoric Tuesday night and how it fits with the facts and political circumstances:

___

OBAMA: "We have subsidized oil companies for a century. That's long enough. It's time to end the taxpayer giveaways to an industry that's rarely been more profitable, and double-down on a clean energy industry that's never been more promising."

THE FACTS: This is at least Obama's third run at stripping subsidies from the oil industry. Back when fellow Democrats formed the House and Senate majorities, he sought $36.5 billion in tax increases on oil and gas companies over the next decade, but Congress largely ignored the request. He called again to end such tax breaks in last year's State of the Union speech. And he's now doing it again, despite facing a wall of opposition from Republicans who want to spur domestic oil and gas production and oppose tax increases generally.

___

OBAMA: "Our health care law relies on a reformed private market, not a government program."

THE FACTS: That's only half true. About half of the more than 30 million uninsured Americans expected to gain coverage through the health care law will be enrolled in a government program. Medicaid, the federal-state program for low-income people, will be expanded starting in 2014 to cover childless adults living near the poverty line.

The other half will be enrolled in private health plans through new state-based insurance markets. But many of them will be receiving federal subsidies to make their premiums more affordable. And that's a government program, too.

Starting in 2014 most Americans will be required to carry health coverage, either through an employer, by buying their own plan, or through a government program.

___

OBAMA, asking Congress to pay for construction projects: "Take the money we're no longer spending at war, use half of it to pay down our debt, and use the rest to do some nation-building right here at home."

THE FACTS: The idea of taking war "savings" to pay for other programs is budgetary sleight of hand. For one thing, the wars in Iraq and Afghanistan have been largely financed through borrowing, so stopping the wars doesn't create a pool of ready cash, just less debt. And the savings appear to be based at least in part on inflated war spending estimates for future years.

___

OBAMA: "Through the power of our diplomacy a world that was once divided about how to deal with Iran's nuclear program now stands as one."

THE FACTS: The world is still divided over how to deal with Iran's disputed nuclear program, and even over whether the nuclear program is a problem at all.

It is true that the U.S., Europe and other nations have agreed to apply the strictest economic sanctions yet on Iran later this year. But the global sanctions net has holes, because some of Iran's large oil trading partners won't go along. China, a major purchaser of Iran's crude, isn't part of the new sanctions and, together with Russia, stopped the United Nations from applying similarly tough penalties.

___

OBAMA: "Tonight, I want to speak about how we move forward, and lay out a blueprint for an economy that's built to last - an economy built on American manufacturing, American energy, skills for American workers, and a renewal of American values."

THE FACTS: Economists do see manufacturing growth as a necessary component of any U.S. recovery. U.S. manufacturing output climbed 0.9 percent in December, the biggest gain since December 2010. Yet Obama's apparent vision of a nation once again propelled by manufacturing ? a vision shared by many Republicans ? may already have slipped into the past.

Over generations, the economy has become ever more driven by services; not since 1975 has the U.S. had a surplus in merchandise trade, which covers trade in goods, including manufactured and farm goods. About 90 percent of American workers are employed in the service sector, a profound shift in the nature of the workforce over many decades.

The overall trade deficit through the first 11 months of 2011 ran at an annual rate of nearly $600 billion, up almost 12 percent from the year before.

___

OBAMA: "The Taliban's momentum has been broken, and some troops in Afghanistan have begun to come home."

THE FACTS: Obama is more sanguine about progress in Afghanistan than his own intelligence apparatus. The latest National Intelligence Estimate on Afghanistan warns that the Taliban will grow stronger, using fledgling talks with the U.S. to gain credibility and stall until U.S. troops leave, while continuing to fight for more territory. The classified assessment, described to The Associated Press by officials who have seen it, says the Afghan government hasn't been able to establish credibility with its people, and predicts the Taliban and warlords will largely control the countryside.

___

OBAMA: "On the day I took office, our auto industry was on the verge of collapse. Some even said we should let it die. With a million jobs at stake, I refused to let that happen. In exchange for help, we demanded responsibility. We got workers and automakers to settle their differences. We got the industry to retool and restructure. Today, General Motors is back on top as the world's number one automaker. Chrysler has grown faster in the U.S. than any major car company. Ford is investing billions in U.S. plants and factories."

THE FACTS: He left out some key details. The bailout of General Motors and Chrysler began under Republican President George W. Bush. Obama picked up the ball, earmarked more money, and finished the job. But Ford never asked for a federal bailout and never got one.

___

OBAMA: "We can also spur energy innovation with new incentives. The differences in this chamber may be too deep right now to pass a comprehensive plan to fight climate change. But there's no reason why Congress shouldn't at least set a clean energy standard that creates a market for innovation."

THE FACTS: With this statement, Obama was renewing a call he made last year to require 80 percent of the nation's electricity to come from clean energy sources by 2035, including nuclear, natural gas and so-called clean coal. He did not put that percentage in his speech but White House background papers show that it remains his goal.

But this Congress has yet to introduce a bill to make that goal a reality, and while legislation may be introduced this year, it is unlikely to become law with a Republican-controlled House that loathes mandates.

___

OBAMA: "Right now, because of loopholes and shelters in the tax code, a quarter of all millionaires pay lower tax rates than millions of middle-class households."

THE FACTS: It's true that a minority of millionaires pay a lower tax rate than some lower-income people. On average, though, wealthy people pay taxes at a much higher rate than middle-income taxpayers.

Obama's claim comes from a Congressional Research Service report that compared federal taxes paid by people making less than $100,000 with those paid by people making more than $1 million. About 10 percent of families with incomes under $100,000 paid more than 26.5 percent in federal income, payroll and corporate taxes. And about a quarter of millionaire taxpayers paid a rate lower than that.

___

OBAMA: "We can't bring back every job that's left our shores.... Tonight, my message to business leaders is simple: Ask yourselves what you can do to bring jobs back to your country, and your country will do everything we can to help you succeed."

FACT CHECK: Many of the jobs U.S. companies have created overseas won't return because they were never in the United States in the first place.

As Obama said in his speech, U.S. workers have become more productive and labor costs have fallen.

But there are powerful forces pushing the other way: Many of the overseas jobs in U.S. companies weren't transferred from the U.S. They were created in fast-growing markets in Latin America, Asia and elsewhere to serve customers in those markets. Companies in the Standard & Poor's 500 index now earn more than half of their revenue from overseas.

That has fueled more job creation abroad. U.S. multinationals cut more than 800,000 jobs in the United States from 2000 to 2009, according the Commerce Department. They added 2.9 million overseas in the same period.

___

OBAMA: "Anyone who tells you that America is in decline or that our influence has waned doesn't know what they're talking about ... That's not how people feel from Tokyo to Berlin; from Cape Town to Rio; where opinions of America are higher than they've been in years."

THE FACTS: Obama left out Arab and Muslim nations, where popular opinion of the U.S. appears to have gone downhill or remained unchanged after the spring 2011 reformist uprisings in the Middle East. A Pew Research Center survey in May found that in predominantly Muslim countries such as Turkey, Jordan and Pakistan, views of the U.S. were worse than a year earlier. In Pakistan, a major recipient of U.S. foreign aid that went unmentioned in Obama's speech, just 11 percent of respondents said they held a positive view of the United States.

___

Associated Press writers Tom Raum, Anne Gearan, Ricardo Alonso-Zaldivar, Martin Crutsinger, Jim Drinkard, Dina Cappiello, Erica Werner, Andrew Taylor, Christopher S. Rugaber and Stephen Ohlemacher contributed to this report.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/89ae8247abe8493fae24405546e9a1aa/Article_2012-01-25-US-State-of-Union-Fact-Check/id-7e14143245054ff6906a6d431fbc2f76

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Turkish film highlights gay honor killing

ANKARA, Turkey (AP) ? Shortly after telling his parents he was gay, Ahmet Yildiz was gunned down inside his car by his father in Istanbul. It was Turkey's first officially recognized gay "honor killing."

An award-winning film partly inspired by Yildiz's story, which opened in dozens of cinemas across Turkey last week, is putting the spotlight on gays in a Muslim country that is seeking European Union membership but remains influenced by conservative and religious values.

The film "Zenne Dancer" ? or male belly dancer ? is not the nation's first gay-themed movie but is the first to explore the little-known phenomenon of men killed by family members for being gay. So-called honor killings in Turkey usually target women accused of disgracing the family.

"Our main aim was to convey Ahmet's story, but by doing so we also wanted to expose the pressure the (gay and lesbian community) faces from their family, the society and the state," said Mehmet Binay, who co-directed and produced the film with his partner, Caner Alper.

"Zenne Dancer" won four awards at Turkey's coveted Antalya Golden Orange film festival this year, including best First Film and Best Cinematography. Erkan Avci, who plays Yildiz's character, won Best Supporting Actor. The movie was also recently chosen a Best Film by an association of Turkish film critics.

Yildiz, a physics student at Istanbul's Marmara University, was shot dead on July 15, 2008 after he went out for an ice cream break while studying at home for his final exams.

An arrest warrant has been issued for his father, Yahya Yildiz, who has been charged in absentia for the murder. The father, who has been on the run for three years, is believed to be in hiding in northern Iraq.

Yildiz, who was a close friend of Binay and Alper, came from the conservative, mostly-Kurdish Sanliurfa province, where homosexuality is taboo and where officials have been struggling to stem the practice of honor killings of women. Women there have been killed for flirting or having a boyfriend without the family's consent.

Gay honor killings are believed to be common in Turkey's conservative heartland. But Yildiz's murder was the first in Turkey to be reported by authorities as a gay honor killing.

Binay said Yildiz's family suspected his homosexuality but believed he could be treated by imams and were pressuring him to return to Sanliurfa.

"He was killed shortly after he told them he would not be cured, would not return and that he was considering leaving for Germany where he might marry (his boyfriend)," he said.

In "Zenne Dancer," Yildiz's life is intertwined with the stories of two other male characters ? a flamboyant Zenne dancer named Can and a bisexual German photographer, Daniel.

A stranger to Turkey's conservative traditions, Daniel encourages Yildiz to come out to his parents, insisting honesty was the best way to deal with his family.

"You don't understand," Yildiz responds in one scene: "Honesty would kill me."

Binay said he and Alper were filming a documentary on male belly dancers when Yildiz was killed. Shocked by the murder, they put the documentary on hold and decided to create a feature film that blends the story of the Zenne dancers with Yildiz's tragedy.

Turkish attitudes toward gay and lesbians are more relaxed compared to the 1980s and 1990s when police routinely raided gay bars, detained transvestites and banned gay festivals. Gay sex is not considered a crime in the country, and some bars and clubs in major cities openly cater to gays.

But a majority of gays still choose to hide their lifestyle in a country where liberal views have yet to make inroads in rural areas and many urban settings.

Last year, a former government minister described homosexuality as a biological disorder that needs to be treated, while municipalities have some leeway to introduce laws safeguarding "morality," which gay activists view as a potential threat to their freedom.

Some gays openly acknowledge their sexual orientation, including poet Murathan Mungan and the late singer Zeki Muren. Zenne dancing itself harks back to the Ottoman Empire, a time when there was a degree of tolerance toward gay sex among some sectors of the elite.

Hebun LGTB, a gay and lesbian group based in the conservative city Diyarbakir that neighbors Sanliurfa, described the film as an opportunity to break ingrained attitudes toward gays in traditional areas.

"There was a piece of us in each of the characters," said a group member, Arif, who declined to give his surname because his family does not know about his sexual orientation. "I am in the same situation as Ahmet Yildiz: If I was honest, I would be killed by my family."

"If out of all the people who watch it, just 10 are able to change their attitudes, then the filmmakers should be happy," he said.

Despite one article in a pro-Islamic newspaper that branded "Zenne Dancer" a "film for perverts," Binay says he and Alper have not received any threats or hate mail, and that some 35,000 people have seen the movie in its first week.

Gulsah Simsek, a 23-year old student, watched "Zenne Dancer" in Ankara.

"Some of the swearing and some of the scenes shocked me," she said. "But there must be so many people like (Yildiz) and it's good that the pain they suffer is being told."

Binay and Alper have been same-sex partners for 14 years and openly came out as a couple during one of the film's early screenings. They regularly attend showings where they hold discussions on attitudes toward homosexuality.

The film is showing in 50 cinemas in 16 out of Turkey's 81 provinces, including conservative Diyarbakir.

"The (positive) response we got in Istanbul wasn't much different to the response we got in Diyarbakir," Binay said. "We are encouraged by the attitudes in (traditional) regions."

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/4e67281c3f754d0696fbfdee0f3f1469/Article_2012-01-24-EU-Turkey-Gay-Killing/id-38160f49904744fea2787a726a2b87b8

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At PSU, tension over ouster, then grief for JoePa (AP)

STATE COLLEGE, Pa. ? Anguished by an unthinkable scandal that shook a university and tarnished the proud football program, many in the Penn State community rallied around a common cause.

They mourned coach Joe Paterno's dismissal and questioned the motives and tactics of school leaders who pushed out the Hall of Famer in November in the wake of child sex abuse charges against a retired assistant coach.

Alumni, fans and students already racked by emotions were jolted by a much greater loss when Paterno died Sunday of lung cancer at age 85 ? and the grieving process again could be complicated following two tense months that often had the Paterno family and the school at odds.

"I feel like from the inside looking out that most people forget that he donated his whole life to the program. ... And everything that he donated to that school, people tend to look over that," defensive end Jack Crawford, who just completed his senior season with the Nittany Lions, said Sunday from Senior Bowl practice in Mobile, Ala.

"It was tough to swallow. It was harder to swallow when he first got fired. It was a sad moment for the whole Penn State family."

A family seemingly torn Nov. 5 after retired defensive coordinator Jerry Sandusky was charged with the first of dozens of counts of abuse allegations. Sandusky has maintained his innocence and is awaiting trial. Paterno testified before a state grand jury investigating Sandusky, and authorities said he wasn't a target of the probe.

It ended up being his undoing anyway.

Paterno fulfilled his legal obligation by reporting a 2002 allegation relayed by a graduate assistant to his university superior. But the state's top cop chastised Paterno, among other school leaders, for failing to fulfill a moral duty to do more and take the allegation to police.

Paterno himself said he "wished he could have done more" when he announced his retirement plans the morning of Nov. 9 before getting ousted by the university Board of Trustees that evening.

"I am saddened to hear the news of Joe Paterno's passing. Joe was a genuinely good person," longtime Nebraska coach and current athletic director Tom Osborne said. "Anybody who knew Joe feels badly about the circumstances. I suspect the emotional turmoil of the last few weeks might have played into it."

That turmoil stretched to Paterno's final days.

Diagnosed with lung cancer days after getting fired, Paterno entered the hospital Jan. 13 for what his family then said was a minor complication from treatments that included radiation and chemotherapy. Mount Nittany Medical Center was barely a half-mile from Beaver Stadium, the Nittany Lions' home field that Paterno helped make into one of college football's shrines during his 46 seasons as Penn State head coach.

While in the hospital, trustees just a couple miles away at a campus hotel on Thursday told of why they fired Paterno and cited in part a failure to fulfill his moral responsibility in connection with the 2002 allegation. His lawyer, Wick Sollers, called the allegations self-serving and reiterated that Paterno fully reported what he knew to the people responsible for campus investigations.

"I think his legacy should be everything wonderful he did here for Penn State and for the community. That's what I hope," Karen Long, 70, of State College, said at the women's basketball game Sunday afternoon between Iowa and Penn State. "I don't think he was treated fairly, though. Just the way they handled firing him was awful."

Against that backdrop, school leaders, the Paterno family and the university community fractured by the scandal appear to be slowly mending relationships.

In recent weeks, university leaders have indicated they intend to honor Paterno's contributions on and off the field ? a sharp contrast to tones sounded in the frantic first week of the scandal. Back then, for instance, school President Rodney Erickson said Paterno was welcome to football games just like any other member of the public.

Paterno won two national championships and a Division I record 409 victories to turn Penn State into a name-brand program. Off the field, Paterno and his wife, Sue, donated millions back to the university, including the library.

"His and Sue's contributions are as much about ensuring student success as the many endowments and the library bearing the Paterno name," said Barbara Dewey, Penn State's dean of University Libraries.

Memorial service and funeral plans weren't ready yet Sunday night, though it appeared the family and the school were coordinating efforts.

Perhaps one last chance to say goodbye for a Penn State community that often took its cues on fall weekends from JoePa.

"No matter what people say, you can't take away what he did for Penn State and college football," former cornerback D'Anton Lynn said. "I don't think there will ever be a college coach that will ever have that impact again."

Source: http://us.rd.yahoo.com/dailynews/rss/topstories/*http%3A//news.yahoo.com/s/ap/20120123/ap_on_sp_co_ne/fbc_paterno_the_final_goodbye

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Michigan Mom Might Have Offered Daughter for Sex at Pawn Shop (ContributorNetwork)

A Monroe, Mich., mother is being investigated for offering a pawn shop employee sexual favors from her daughter in exchange for dropping a $25 extension fee on her $120 laptop payment, says My Fox Detroit. Here are details about this case.

* Al Hassan, an employee at DaSilva's Pawn Shop in Southgate, Mich., contacted police after a customer offered to perform a sex act on him when she couldn't pay the $25 extension on the laptop.

* According to Fox Detroit, the woman, whose name is being withheld, said: "What about me and my daughter can do something for you in the back room?"

* Store surveillance cameras showed the woman in the store and her daughter playing and jumping around. When the mother mentioned the proposition, the clerk reported the daughter said, "Not me this time, Mom. You mean you."

* Police investigated the 36-year-old woman's townhouse and found the 10-year-old child in no immediate danger. As of Wednesday, the News Herald reports the child has not been removed from the home.

* The mother admitted to police she had promised the clerk a sexual favor if he would overlook the deposit owed on the laptop but adds she was joking and she had not included her daughter in the offer.

* The mother stated the clerk probably misunderstood her because of the way she phrased the offer but she didn't say she was offering sex from her daughter.

* In an interview with News 4, the mother denies doing anything wrong. She calls the offer "flirting" but that she wasn't serious. "I don't want to be seen as a bad mom because I needed the $25 for gas and groceries."

* Child Protective Services is following up with the mother and child, but a supervisor told USA Today that confidentiality laws prevent case workers from discussing details of the investigation.

* Police are still trying to determine if the mother's comment was a misunderstanding or a blatant proposition. The video camera only places the woman and child in the store; it does not give them access to the verbal exchange or any corroborating witnesses.

* Det. Lt. Edward Sukel told the News Herald that, "we're cautiously moving forward because, of course, we want to make sure we know what we have and not make any rash decisions."

Marilisa Kinney Sachteleben writes about people, places, events and issues in her native state of "Pure Michigan."

Source: http://us.rd.yahoo.com/dailynews/rss/personaltech/*http%3A//news.yahoo.com/s/ac/20120122/us_ac/10865753_michigan_mom_might_have_offered_daughter_for_sex_at_pawn_shop

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Tuesday, January 24, 2012

Investing In Haiti: Challenges, Strategies, and Opportunities | Tufts ...

Dear friends,

You are cordially invited to BrandHaiti?s Second Annual Business Symposium entitled ?Investing in Haiti: Challenges, Strategies, Opportunities?! This unique event will take place in Cabot Auditorium at the Fletcher School at Tufts University Saturday, February 11th, from 10:30AM to 5:00PM (ET). REGISTER NOW! The purpose of the symposium is to bring the entrepreneurial potential of Haiti to the forefront of economic policy while simultaneously deconstructing the negative conceptions too often associated with the country. This symposium will assemble prominent members of the business community, students, activists, and organizations that currently work to ameliorate the Haitian economy, but who will also discuss the promising future for a sovereign Haitian with an innovative private sector. It is our hope that you will share your vast personal and professional experiences with the students, academics, activists, economists, and investors in attendance during the question and answer sessions of our four panels:

1. Entrepreneurship

2. Investment Industries

3. Infrastructure & Economic Development

4.The Role of the Diaspora in Promoting Growth

BrandHaiti is a non-profit, student-led marketing organization that re-brands the negative images and perceptions of Haiti currently dominating international discourse. Our goal is to highlight the Haitian people?s resiliency, creative capacity, and entrepreneurial ability to foster pro-Haitian business investment and revitalize a non-exploitative economy. We do so through a number activities, including the creation of a product line to connect Haitian artisans and entrepreneurs directly to markets, an inaugural spring break program to expose young foreign entrepreneurs to the beautiful Caribbean island of Haiti and network with Haitians, expos?s of Haitian entrepreneurs on our website with photos and videos to highlight the positive aspects of doing business in Haiti, and many more.

Currently, here is list of confirmed speakers:

?????? Drusillia Brown, Economist at Tufts and Chair of International Relations Studies
?????? Prof. Bernard Simonin, Nation-Branding Specialist at Fletcher School
?????? Genevieve Lemke, Owner of Wahoo Bay Beach Club & Resort in Haiti
?????? Joey Adler, CEO of Diesel and Founder of ONEXONE
?????? Jean-Patrick Lucien, CEO of BelSoley
?????? Fritz Charles, Haiti Ventures
?????? Mathias Pierre, Executive Director of GaMa Consulting Inc. in Haiti
?????? Jacques-Philippe Piverger, CEO of Global Syndicate
?????? Wilhelm Lemke, CEO of d?Adesky Group
?????? Legrand Gandhi Lindor, Esq., CEO of LGappareL, LLC, President of Provincial Real Estate Group
?????? Chef Ron DuPrat, ?Top Chef? and Culinary School of Haiti
?Top Chef? Ron DuPrat has generously agreed to cater the event. This year?s symposium is generously sponsored by the Institute for Global Leadership at Tufts University.

It is our hope you will join us in an effort to construct practical solutions to investing in Haiti, especially investing in those young Haitian entrepreneurs who are the future of this Caribbean nation. REGISTER TODAY!

Best regards,

The BrandHaiti Team

Source: http://www.trcommons.org/2012/01/investing-in-haiti-challenges-strategies-and-opportunities/

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How protein networks stabilize muscle fibers: Same mechanism as for DNA

How protein networks stabilize muscle fibers: Same mechanism as for DNA [ Back to EurekAlert! ] Public release date: 23-Jan-2012
[ | E-mail | Share Share ]

Contact: Dr. Wolfgang Linke
wolfgang.linke@rub.de
49-234-322-9101
Ruhr-University Bochum

Research team with RUB involvement reports in Genes and Development

The same mechanism that stabilises the DNA in the cell nucleus is also important for the structure and function of vertebrate muscle cells. This has been established by RUB-researchers led by Prof. Dr. Wolfgang Linke (Institute of Physiology) in cooperation with American and German colleagues. An enzyme attaches a methyl group to the protein Hsp90, which then forms a complex with the muscle protein titin. When the researchers disrupted this protein network through genetic manipulation in zebrafish the muscle structure partly disintegrated. The scientists have thus shown that methylation also plays a significant role outside the nucleus. They published their results in Genes and Development.

Methylation in the nucleus

Enzymes, called methyltransferases, transfer methyl (CH3) groups to specific sections of the DNA in the nucleus. In this way, they mark active and inactive regions of the genes. However, not only DNA but also nuclear proteins incur methylation, mostly at the amino acid lysine. Methylated lysines on nuclear proteins promote the formation of protein complexes that control, for example, DNA repair and replication. However, methyltransferases are not only found in the nucleus, but also in the cellular fluid (cytoplasm). Yet, it is not well established which proteins they methylate in the cytoplasm and how this methylation may affect function.

Shown for the first time: methylation in the cytoplasm promotes protein complex formation

The researchers first identified an enzyme which is mainly present in the cytoplasm and which methylates the amino acid lysine (Smyd2). Then they searched for interaction partners of the enzyme Smyd2 and found the heat shock protein Hsp90. The scientists went on to show that Smyd2 and methylated Hsp90 form a complex with the muscle protein titin. "Titin is the largest protein in the human body and known primarily for its role as an elastic spring in muscle cells" explains Linke. "Precisely this elastic region of titin is protected by the association with methylated Hsp90."

Titin requires protection by methylated proteins

In skeletal muscle cells of the zebrafish, Linke's team explored what happens when the protection by the methylated heat shock protein is repressed. By genetic manipulation they altered the organism in such a way that it no longer produced the enzyme Smyd2, which blocked the methylation of Hsp90. Without methylated Hsp90, the elastic titin region was unstable and muscle function strongly impaired; the regular muscle structure was partially disrupted.

###

Bibliographic record

L.T. Donlin, C. Andresen, S. Just, E. Rudensky, C.T. Pappas, M. Kruger, E.Y. Jacobs, A. Unger, A. Zieseniss, M.-W. Dobenecker, T. Voelkel, B.T. Chait, C.C. Gregorio, W. Rottbauer, A. Tarakhovsky, W.A. Linke (2012): Smyd2 controls cytoplasmic lysine methylation of Hsp90 and myofilament organization, Genes and Development, doi: 10.1101/gad.177758.111

Further information

Prof. Dr. Wolfgang Linke, Department of Cardiovascular Physiology, Institute of Physiology, Faculty of Medicine at the Ruhr-Universitt, 44780 Bochum, Germany, Phone: +49/234/32-29101
wolfgang.linke@rub.de

Click for more

Cardiovascular Physiology at RUB
http://www.py.ruhr-uni-bochum.de/kardp/Index.html.en

Figure online

A figure related to this press release can be found online at: http://aktuell.ruhr-uni-bochum.de/pm2012/pm00017.html.en

Editor

Dr. Julia Weiler


[ Back to EurekAlert! ] [ | E-mail | Share Share ]

?


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How protein networks stabilize muscle fibers: Same mechanism as for DNA [ Back to EurekAlert! ] Public release date: 23-Jan-2012
[ | E-mail | Share Share ]

Contact: Dr. Wolfgang Linke
wolfgang.linke@rub.de
49-234-322-9101
Ruhr-University Bochum

Research team with RUB involvement reports in Genes and Development

The same mechanism that stabilises the DNA in the cell nucleus is also important for the structure and function of vertebrate muscle cells. This has been established by RUB-researchers led by Prof. Dr. Wolfgang Linke (Institute of Physiology) in cooperation with American and German colleagues. An enzyme attaches a methyl group to the protein Hsp90, which then forms a complex with the muscle protein titin. When the researchers disrupted this protein network through genetic manipulation in zebrafish the muscle structure partly disintegrated. The scientists have thus shown that methylation also plays a significant role outside the nucleus. They published their results in Genes and Development.

Methylation in the nucleus

Enzymes, called methyltransferases, transfer methyl (CH3) groups to specific sections of the DNA in the nucleus. In this way, they mark active and inactive regions of the genes. However, not only DNA but also nuclear proteins incur methylation, mostly at the amino acid lysine. Methylated lysines on nuclear proteins promote the formation of protein complexes that control, for example, DNA repair and replication. However, methyltransferases are not only found in the nucleus, but also in the cellular fluid (cytoplasm). Yet, it is not well established which proteins they methylate in the cytoplasm and how this methylation may affect function.

Shown for the first time: methylation in the cytoplasm promotes protein complex formation

The researchers first identified an enzyme which is mainly present in the cytoplasm and which methylates the amino acid lysine (Smyd2). Then they searched for interaction partners of the enzyme Smyd2 and found the heat shock protein Hsp90. The scientists went on to show that Smyd2 and methylated Hsp90 form a complex with the muscle protein titin. "Titin is the largest protein in the human body and known primarily for its role as an elastic spring in muscle cells" explains Linke. "Precisely this elastic region of titin is protected by the association with methylated Hsp90."

Titin requires protection by methylated proteins

In skeletal muscle cells of the zebrafish, Linke's team explored what happens when the protection by the methylated heat shock protein is repressed. By genetic manipulation they altered the organism in such a way that it no longer produced the enzyme Smyd2, which blocked the methylation of Hsp90. Without methylated Hsp90, the elastic titin region was unstable and muscle function strongly impaired; the regular muscle structure was partially disrupted.

###

Bibliographic record

L.T. Donlin, C. Andresen, S. Just, E. Rudensky, C.T. Pappas, M. Kruger, E.Y. Jacobs, A. Unger, A. Zieseniss, M.-W. Dobenecker, T. Voelkel, B.T. Chait, C.C. Gregorio, W. Rottbauer, A. Tarakhovsky, W.A. Linke (2012): Smyd2 controls cytoplasmic lysine methylation of Hsp90 and myofilament organization, Genes and Development, doi: 10.1101/gad.177758.111

Further information

Prof. Dr. Wolfgang Linke, Department of Cardiovascular Physiology, Institute of Physiology, Faculty of Medicine at the Ruhr-Universitt, 44780 Bochum, Germany, Phone: +49/234/32-29101
wolfgang.linke@rub.de

Click for more

Cardiovascular Physiology at RUB
http://www.py.ruhr-uni-bochum.de/kardp/Index.html.en

Figure online

A figure related to this press release can be found online at: http://aktuell.ruhr-uni-bochum.de/pm2012/pm00017.html.en

Editor

Dr. Julia Weiler


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Source: http://www.eurekalert.org/pub_releases/2012-01/rb-hpn012312.php

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Monday, January 23, 2012

Decide on an Automotive Oscilloscope Fit to you | Edimaster

Quite a few persons will undoubtedly be baffled about learn how to pick a really good automotive diagnostic tool. A lot of people like the experts from the fields of automotive fix and electrical engineering typically need the ideal diagnostic oscilloscope. They need it to do their work perfectly. To get the ideal automotive oscilloscope, you?ll want to identify it suited for your expectations.

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They are a large number of automotive diagnostics to suit your needs to choose in our shop. Automotive Diagnostic tool is definitely the trend from the subject of oscilloscope. Now days, portable oscilloscope is required and come to be popular in our each day daily life. We want the automotive diagnostics can come in hand and portable adequate to become moved just about everywhere. Oscilloscope is probably the examples in the outcome of this growing request of portable products. Typically the portable oscilloscope has large sensitivity, best tool for provider, convince and another form reasons. This sort value the money you charge on it. If you want to order a diagnostic tool could report the waveform without difficulty in addition to the portable oscilloscope is definitely a good choice. Right here have various varieties in the portable oscilloscope. Hope you might discover a sort suited to you personally. The final good tip to suit your needs: Compare diverse programs and pick a suitable a person for your auto well before obtain site it.

We suggested to you personally that to choose a a person happens using a money-back guarantee. Also, invest in the a person with return policy and try to guarantee which the diagnostic tool equipment can function adequately. As to the applications and product?s upgrades assist, you possibly can question the vendor. will undoubtedly be on your choice.

Source: http://www.edimaster.org/decide-on-an-automotive-oscilloscope-fit-to-you/

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